Fig. 6

Predicted locations of potential bacterial lipid transfer proteins. Locations predicted from analysis of hydrophobic N-termini and acylation signals for: a DUF2140, TULIP in Gram +ve bacteria; b TULIPs in Gram –ve bacteria: DUF4403, YceB/DUF1439 and Rv0817c/DUF2993 (c) TamB in Gram –ve bacteria such as E. coli, with N-terminus homologous to Chorein-N and C-terminus forming a narrower hydrophobic tubular “β-taco”. All dimensions, including the inter-membrane space 20 nm across as in E. coli, lengths of TULIP domains, location and length of linkers, and membrane anchors are drawn to approximate scale, except the hydrophobic seams/openings, which are enlarged and drawn straight, compared to narrower helical openings found in nature. Shading: cytoplasm blue, inner membrane pink, intermembrane space yellow, outer membrane green. The three different types of domain with hydrophobic cavities are shown in a key at the bottom