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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Proton pump inhibitors can reverse the YAP mediated paclitaxel resistance in epithelial ovarian cancer

Fig. 2

YAP-TEAD1 confers PTX-resistance to EOC cells. Cells were treated with different concentrations (0-23 μmol/L) of PTX for 48 h. a Dose-response curve of PTX-sensitive and PTX-resistant EOC cells. b The 50% maximal inhibitory concentration (IC50) values of PTX in the PTX-sensitive parent cell line A2780 and its resistant clone A2780/T were calculated. Each value represents the mean ± SD from three independent experiments (**P < 0.01). c YAP, p-YAP, Cyr61 and P-gp protein levels were determined by western blot. d-e Western blotting analyses of YAP, p-YAP, Cyr61 and P-gp protein expression in YAP-low/ PTX-sensitive A2780 cells and in YAP-high/PTX-resistant A2780/T cells by plasmids expressing His-tagged YAP and YAP siRNA stable transfection, respectively. CCK-8 assay indicated that over-expression of YAP in A2780 cells significantly reduced its drug-sensitivity to PTX while knocked down of YAP in A2780/T cells sensitized their response to PTX treatments. Each value represents the mean ± SD from three independent experiments (**P < 0.01). f Western blotting analyses of TEAD1 protein expression in A2780 and A2780/T cells. g Activation of CTGF reporter by YAP and TEAD1. A luciferase reporter driven by CTGF promoter was co-transfected with YAP wild type or S94A mutant as indicated with or without TEAD1 co-transfection. Luciferase activity was measured and normalized to co-transfected β-galactosidase. h Dominant-negative TEAD1 blocks the YAP stimulation of the CTGF reporter. The indicated plasmids were co-transfected, and luciferase activity was determined as in G

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