Fig. 4

PPI reduced PTX resistance in EOC cells through the inhibition of YAP. Synergetic effects of the combination of PPI and PTX resulted in the inhibition of YAP and cytotoxicity to PTX-resistant EOC cells. Two EOC cells (A2780 and A2780/T) were treated with different concentrations of either PTX or combinated with sub-lethal doses of EMSO (130 and 150 μM) for 48 h. a Dose-response curve of EMSO treatment in the two EOC cell lines. b Cell viability is shown after PTX treatments with or without EMSO for 48 h using CCK-8 assay in PTX-sensitive A2780 cells and PTX-resistant A2780/T cells. Each value represents the mean ± SD from three independent experiments. c The mRNA level of MDR1, Cyr61 and YAP were determined using a real-time PCR kit. Data represent mean ± SD from three independent experiments (**P < 0.01). d The expressions of p-gp, TEAD1, YAP and p-YAP protein were detected by Western blot analysis. e YAP expression on the cytoplasm and nuclear of cells is shown using green staining. Cell immunofluorescence was detected by confocal microscopy. Magnification × 600; Nuclear staining with DAPI is shown in blue. Representative images from three independent experiments are shown. f The expressions of p-AKT was detected by Western blot