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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Proton pump inhibitors can reverse the YAP mediated paclitaxel resistance in epithelial ovarian cancer

Fig. 5

PPI-mediated apoptosis was revealed using a caspase-independent apoptotic-like cytotoxicity upon PTX treatment of EOC cells. a Quantification of apoptotic cells: The apoptosis ratio is the ratio of the number of cells in early-stage apoptosis to that in advanced stage apoptosis. Data are presented as the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01). b Images of fluorescent cells were taken after staining with Hoechst-33342 following treatment with EMSO (130 or 150 μM), PTX (0.25 or 2 μM), PTX (0.25 or 2 μM) + EMSO (130 or 150 μM) for 48 h. The white arrows denote early-stage apoptotic nuclei (× 400). c Expression of active caspase-3 and caspase-8, as measured by an ELISA assay. Data (mean ± SD) are from three independent experiments. d Cytograms obtained for A2780 and A2780/T cells following a 48 h- continuous exposure to EMSO (130 or 150 μM), or PTX (0.25 or 2 μM) + EMSO (130 or 150 μM), in the absence and presence of Z-VAD-fmk

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