Fig. 5

miR-21 inhibited the expression of caspase-8 in Act-HaCaT and Act-HHEK. a Prediction of the binding site between miR-21 and caspase-8; b Transfection with miR-21 mimic and caspase-8 WT decreased the relative luciferase activity (miR-21/miR-NC); c Transfection with miR-21 mimic significantly decreased the expression of caspase-8; d The expression of caspase-8 measured by western blotting after transfection with miR-21 inhibitor, miR-21 mimic, caspase-8 mRNA, and caspase-8 shRNA, respectively. All the results were shown as mean ± SD (n = 3), and the experiments were repeated three times