Skip to main content
Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: lncRNA H19 promotes matrix mineralization through up-regulating IGF1 by sponging miR-185-5p in osteoblasts

Fig. 1

The expression of lncRNA H19 and miR-185-5p in mineralized osteoblasts. a The results of Alizarin Red staining, mineralization nodules and ralative areas at 7, 14 and 21 day after induced mineralization of MC3T3-E1 cells. Scale bars, 800 μm. b, c The mRNA expression of OCN, ALP and Collagen I in control and mineralized MC3T3-E1 cells were assessed by qPCR and western blot on day 7. GAPDH was used for normalization. d The expression of H19, IGF1 and miR-185-5p in control and mineralized MC3T3-E1 cells were detected by qPCR. GAPDH or U6 was used for normalization, respectively. The asterisks show difference significant as ** p < 0.01, * p < 0.05

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us