Fig. 2

H19 knockdown inhibits matrix mineralization of osteoblasts. a, b The mRNA expression of OCN, ALP and Collagen I in control and H19 knockdown group of MC3T3-E1 cells were analysed by qPCR and western blot. GAPDH was used for normalization. c ELISA analysis of the amount of Collagen I and BALP protein in the supernatant of MC3T3-E1 cells after treatment with NC and H19 siRNA. d Representative images of ALP staining of MC3T3-E1 cells on day 7 after treatment with NC and H19 siRNA. Scale bars, 800 μm. e Staining of calcium deposition by Alizarin Red in MC3T3-E1 cells on day 14 treated with si-NC and si-H19. Scale bars, 800 μm. The asterisks show difference significant as ** p < 0.01, * p < 0.05