Fig. 3

H19 acts as a ceRNA by sponging miR-185-5p and regulated IGF1 expression indirectly. a The expression level of miR-185-5p in H19 knockdown treated MC3T3-E1 cells by qPCR. U6 was used for normalization. b The predicted binding site between the lncRNA H19 and miR-185-5p by starBase. Mus musculus: mmu. c The luciferase activity of the H19-WT and H19-MUT in cells treated with miR-185-5p mimics or miR-NC. d The predicted binding site between the miR-185-5p and IGF1 by starBase. e The luciferase activity of the IGF1-WT and IGF1-MUT in cells treated with miR-185-5p mimics or miR-NC. f The expression level of IGF1 in miR-185-5p mimics treated cells by qPCR. The asterisks show difference significant as ** p < 0.01, * p < 0.05