Fig. 4

miR-185-5p overexpression inhibits matrix mineralization of osteoblasts. a The expression of IGF1 in MC3T3-E1 cells transfected with miR-185-5p mimics and miR-NC by western blot. GAPDH was used for normalization. b The mRNA expression of OCN, ALP and Collagen I in in MC3T3-E1 cells transfected with miR-185-5p and NC by qPCR and western blot. GAPDH was used for normalization. c ELISA analysis of the amount of Collagen I and BALP protein in the supernatant of MC3T3-E1 cells. d Representative images of ALP staining of MC3T3-E1 cells after transfection. Scale bars, 800 μm. e Staining of calcium deposition by Alizarin Red in MC3T3-E1 cells after treatment with miR-NC and miR-185-5p mimics. Scale bars, 800 μm. The asterisks show difference significant as ** p < 0.01, * p < 0.05