Skip to main content

Advertisement

Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Construction of strains to identify novel factors for regulation of centromeric cohesion protection (CCP) and sister kinetochore mono-orientation (SKM)

Fig. 1

Construction of engineered strains. a Diagrammatic representation of pESC-URA vector. b Construct cloned in pESC-URA along with the gal promoter and corresponding terminator. The black arrow represents the site which was amplified by PCR for the cloning in the pRS406 integration vector. c Restriction digestion of pRS406 constructs using HindIII and NotI. L stands for ladder, lane 1 represent the digestion of pRS406-GAL-MAM1 construct similarly 2, 3, 4, 5 and 6 represent the pRS406-GAL-CDC5, pRS406-GAL-CDC5-MAM1, pRS406-GAL-SPO13, pRS406-GAL-REC8, pRS406-GAL-SPO13-REC8 respectively. d CRY1 strain was transformed with pESC-GAL-CDC5-MAM1-URA (GAL-Cdc5-Mam1), pESC-GAL-SPO13-REC8-URA (GAL-Rec8-Spo13) and pESC-URA (c) construct and single colony was picked and checked for the expression of these proteins at different time point after galactose induction as described in methodology

Back to article page