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Table 2 List of the strains prepared and used in this study

From: Construction of strains to identify novel factors for regulation of centromeric cohesion protection (CCP) and sister kinetochore mono-orientation (SKM)

Strain ID Nick Name Genotype Background
SGY229 WT CENV-GFP Mat-a, leu2::tetR-GFP::LEU2::TetO-HIS3 CRY1
SGY4104 CRY1-pRS406-control Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL -URA CRY1
SGY4105 CRY1-pRS406-GAL-CDC5 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL -CDC5-URA CRY1
SGY4106 CRY1-pRS406-GAL-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - MAM1-URA CRY1
SGY4107 CRY1-pRS406-GAL-CDC4-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - CDC4-MAM1-URA CRY1
SGY4108 CRY1-pRS406-GAL-REC8 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - REC8-URA CRY1
SGY4109 CRY1-pRS406-GAL-SPO13 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - SPO13-URA CRY1
SGY4110 CRY1-pRS406-GAL-REC8-SPO13 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - REC8-SPO13-URA CRY1
SGY4111 CRY1-pRS405 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU CRY1
SGY4112 CRY1-pRS405-GAL-SPO13 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-SPO13-LEU CRY1
SGY4113 CRY1-pRS405-GAL-CDC5 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU CRY1
SGY4114 CRY1-pRS405-GAL-CDC5-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU CRY1
SGY4119 CRY1-pRS405-GAL-SPO13-REC8 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-SPO13-REC8-LEU CRY1
SGY4129 Cdc16–123-pRS405 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU Cdc16–1
SGY4130 Cdc16–123-pRS405-CDC5 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU Cdc16–1
SGY4131 Cdc16–123-pRS405-CDC5-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU Cdc16–1
SGY4132 Cdc16–1-pRS405 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU Cdc16–123
SGY4133 Cdc16–1-pRS405-CDC5 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU Cdc16–123
SGY4134 Cdc16–1-pRS405-CDC5-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU Cdc16–123
SGY4135 Cdc20–1-pRS405 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU Cdc20–1
SGY4136 Cdc20–1-pRS405-CDC5 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU Cdc20–1
SGY4137 Cdc20–1-pRS405-CDC5-MAM1 Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU Cdc20–1