Fig. 3
From: Design and structural characterisation of olfactomedin-1 variants as tools for functional studies
Preparative and analytical SEC profiles of the three Olfm1 constructs confirm their predicted oligomeric state a SEC chromatogram of Olfm1Olf on a HiLoad 16/60 Superdex75 column. b SEC chromatogram of Olfm1coil-Olf on a HiLoad 16/60 Superdex200 column, corresponding to the second injection (see Materials and methods section for details). c SEC chromatogram of Olfm1BMY on a HiLoad 16/60 Superdex200 column. An accolade indicates the pooled fractions for all three preparative chromatograms. d Analytical SEC-MALS analysis on a Superdex200 10/300 increase column of Olfm1Olf, Olfm1coil-Olf and Olfm1BMY confirms their predicted respective monomeric, dimer and tetrameric states (colours corresponding to panels a, b and c, respectively). The standard, aldolase (158 kDa), is shown in yellow and was used to calibrate the MALS signal