Fig. 2

Characterisation of CB-MSC senescence markers under normoglycaemic and hyperglycaemic conditions. a Telomere length analysis for CB-MSCs at PD15, PD50, PD100 and PD200, determined by Southern blotting. Right and left hand lanes represent separated DIG-labelled telomere length standards (kb, in Kit). CTRL represent telomere length positive control (in Kit). b Mean telomere lengths calculated for CB-MSCs at PD15, PD50, PD100 and PD200, with values ± SE indicated. c Telomere maintenance marker, rTERT and TR, expression at PD15, PD50, PD100 and PD200 under normoglycaemic and hyperglycaemic conditions, by RT-PCR. d Percentage SA-β-galactosidase positive CB-MSCs at PD15, PD50, PD100 and PD200 under normoglycaemic and hyperglycaemic conditions, demonstrating low (< 3%) positive staining in CB-MSC populations overall. Significance between CB-MSC populations are shown, ***p < 0.001, **p < 0.01, *p < 0.05