Fig. 4

Characterisation of CB-MSC stem cell properties under normoglycaemic and hyperglycaemic conditions. a CB-MSCs at PD15, PD50, PD100 and PD200 under normoglycaemic and hyperglycaemic conditions, expressed CD73, CD90 and CD105; and were negative for CD45. CD105 expression appeared lost at PD200 under hyperglycaemic conditions. CB-MSCs also expressed low levels of CD34 throughout culture expansion, irrespective of normoglycaemic/hyperglycaemic conditions. CD146, Slug and Snail were expressed throughout expansion under normoglycaemic/hyperglycaemic conditions. CD106, Nanog and Oct4 expression were undetectable. b Colony forming efficiencies (CFEs) for CB-MSCs at PD15 had significantly lower efficiencies, compared to PD50, PD100 and PD200, in both normoglycaemic and hyperglycaemic conditions. CFEs at PD50 and PD100 were significantly higher under hyperglycaemic, compared to normoglycaemic conditions (days 3–5). CFEs were significant decreased at PD200 by hyperglycaemia, compared to normoglycaemic conditions (days 4–5). Significance between CB-MSC populations are shown, ***p < 0.001, **p < 0.01, *p < 0.05