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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Detection of 8-oxoguanine and apurinic/apyrimidinic sites using a fluorophore-labeled probe with cell-penetrating ability

Fig. 4

Cellular localization and uptake of the TAT-S3 probe. a HeLa cells were treated with the TAT-S3 probe for 24 h and then treated with 100, 500, and 1000 μM H2O2 for 1 h. HeLa cells were stained with Mitotracker (Green) at 37 °C for 15 min and then stained with Hoechst (Blue) at 37 °C for 5 min. The staining intensity was observed by confocal laser scanning microscope. b HeLa cells were treated the TAT-S3 probe (2 μM) for 24 h and then treated with 500 μM H2O2 for 1 h. HeLa cells were sonicated and centrifuged to obtain three fractions: Nuclear (N), Mitochondrial (M) and Cytoplasmic (c). Fluorescence was measured on a fluorescence microplate reader at 685/709 nm. Data shown represent the means of 3 individual experiments. c HeLa cells were treated with the TAT-S3 probe for 24 h and then treated with 500 μM H2O2 for 1 h. HeLa cells were stained with Mitotracker at 37 °C for 15 min. The intensities of the TAT-S3 probe (Red) and Mitotracker (Green) were detected using flow cytometry. d HeLa cells were treated with the TAT-S3 probe for 24 h and then treated with 1000 μM H2O2 for 1 h. HeLa cells were stained with Mitotracker at 37 °C for 15 min. The intensities of the TAT-S3 probe (Red) and Mitotracker (Green) were detected using flow cytometry

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