Fig. 2
From: Binding of Brucella protein, Bp26, to select extracellular matrix molecules
Binding of Bp26 protein to soluble ECM components. Representation of different concentrations of collagen type I (a), fibronectin (b), and vitronectin (c) after incubation with immobilized Bp26 protein (5 μg/ μl) on ELISA microtiter plates and detection of binding affinity. Binding of the molecules was detected by peroxidase reaction using MABs specific for each molecule, followed by peroxidase conjugate and substrate as detailed in Materials & Methods. The optical density values of the negative controls were subtracted from the binding values. Each value represents the mean ± SD for each of four independent tests. The ECM molecules concentrations are expressed in μg ml − 1