Fig. 5
From: Binding of Brucella protein, Bp26, to select extracellular matrix molecules
Mapping of binding sites to Bp26 structure. a Structure of channel-like hexadecameric Bp26 shown in cartoon representation. Each monomer is coloured differently. b Binding sites for mAb mapped on Bp26 monomer structure. Regions corresponding to high-binding peptides from Fig. 4a are shown in red. c Binding sites for collagen mapped on Bp26 monomer structure. Regions corresponding to high-binding peptides from Fig. 4b are shown in red. d Binding sites for vitronectin mapped on the Bp26 monomer structure. Regions corresponding to high-binding peptides from Fig. 4c are shown in red. e Binding sites for collagen mapped onto hexadecameric Bp26 complex. The structure is viewed from the side on the right and from the bottom (i.e. through the channel) on the right. The regions corresponding to the high-binding peptides from Fig. 4b have been coloured in red for the monomers in the lower half of the complex. The structure shows the C-termini of the proteins are arranged along the rim of the channel. All structures in the figure were prepared with PyMOL (Schroedinger) and are based on the Bp26 crystal structure (PDB ID: 4HVZ)