Fig. 10

DUF3669-KRAB-ZNF protein complexes and DNA binding signatures a. DUF-KRAB-ZNF protein-protein interaction network taking into account only ZNF proteins found in protein complexes with any DUF3669-KRAB-ZNF protein. Arrows point from bait to prey. Line width reflects detection in one or two (thicker line) studies. Derived from data that were based on affinity-purification/mass spectrometry using tagged DUF3669-KRAB-ZNF proteins (Bioplex 2.0, [41, 51], a yeast two-hybrid system [52] and co-immunoprecipitation [53] and including our ZNF746/ZNF777 data). b. Zinc finger “DNA binding code” signatures for the DUF3669-KRAB-ZNF ortholog groups (see Methods). Each ortholog group depiction relied on the alignment of the individual signatures from all ortholog sequences (count given to the right). The zinc fingers (ZF) are numbered according to the human member. The horizontal placement of the different group signatures indicates some homologies between the whole paralog group. c. Heatmap describing the overlap between the genomic DNA binding sites based on the Jaccard statistic. We included available data for six DUF3669-KRAB-ZNF proteins and for the three ZNF proteins with the lowest and three with the highest number of binding sites in the dataset from [32] for comparison. The DUF3669-KRAB-ZNF proteins are labeled in red. The numbers of genomic binding sites are listed in brackets to the right of the protein name. See Methods for details and the included datasets