Fig. 4

Dependency of the transcriptional repressor functions of ZNF746 and ZNF777 on TRIM28 and SETDB1. Results of dual luciferase reporter assays using Gal4 DNA-binding domain fusions of the indicated protein segments. The ZNF10 KRAB domain (Z10AB) served as positive control for dependency on TRIM28 and SETDB1. a ZNF746a-derived protein fragments. b ZNF746b-derived protein fragments. c ZNF777-derived protein fragments. Bar plots depict normalized mean repression factor values ± STDEV of at least six biological replicates from three independent experiments relative to experiment-specific and cell-type specific values for the Gal4 DNA-binding domain alone. Results for wildtype HAP1 cells (HAP1-WT; blue bars), TRIM28-knockout HAP1 cells (TRIM28ko, orange bars) and SETDB1 knockout HAP1 cells (SETDB1ko, grey bars). Asterisks indicate results of a two-tailed unpaired t-test (* p < 0.05, ** p < 0.01, *** p < 0.001; n.s. = no statistical significance, i.e. p > 0.05) when comparing knockout with wildtype data of a protein fragment