Fig. 7

Analysis of transferability of DUF3669 and the potential impact of sumoylation. a Negligible impact of DUF3669 domains from ZNF746a and ZNF777 on the repressor activity of ZNF10 KRAB-AB. Wildtype ZNF10-AB and fusions of ZNF10-AB to DUF3669 domain segments from ZNF746a or ZNF777 were analyzed by dual luciferase assays in HAP1 wildtype, SETDB1 and TRIM28 knockout cells as in Fig. 4. b Mutation of a major SUMO-acceptor lysine (K189R) in the Z746a/1–279 fragment abolished its repressor activity in HeLa cells (blue bars) and HAP1 wildtype cells (orange bars). Bars visualize mean repression factor values ± STDEV of at least six biological samples from three independent experiments. Asterisks indicate results of a two-tailed unpaired t-test (*** p < 0.001; n.s. = not significant, i.e. p > 0.05) for the comparison of the DUF3669 fusions with the KRAB domain of ZNF10 (Z10AB)