Fig. 9

In vitro evaluation of homo- and hetero-dimerization of DUF3669-containing polypeptides of ZNF746 and ZNF777. Crude bacterial extracts containing the indicated recombinant proteins were mixed and used to pull-down His-tagged proteins. Western blots were probed with anti-His tag antibodies and either GST (the four panels to the left) or MBP (the two panels to the right) tag antibodies to visualize the proteins in the input extracts (lanes “X”) and to identify the enriched recombinant proteins. GST alone and MBP alone were used as negative controls. Images represent black/white overlays of the original two color channels. Grey arrows denote His-tagged proteins, white arrows to GST and GST fusion bands and black arrows to MBP and MBP fusion proteins