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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Heavy chain dimers stabilized by disulfide bonds are required to promote in vitro assembly of trastuzumab

Fig. 3

Gel SDS-PAGE of refolding sequence of LC and HC. a. LC refolding process. M: molecular marker; Sx: Superdex peak of denatured and reduced LC; Conc: concentrated; Dia: LC diafiltered by slow dialysis; FT: flow-through of Capto L; Peak: Capto L elution peak. b. HC refolding process. M: molecular marker; i: Superdex peak of denatured and reduced HC; dia: HC diafiltered by slow dialysis; FT: flow-through of MAb Select SURE; peak: MAb Select SURE elution peak

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