Table 2 The qPCR primer pairs for detecting ISG genes
Genes | Primer pairs | |
|---|---|---|
Forward | Reverse | |
IRF9 | gccctacaaggtgtatcagttg | tgctgtcgctttgatggtact |
IRF7 | gctggacgtgaccatcatgta | gggccgtataggaacgtgc |
PKR | gccgctaaacttgcatatcttca | tcacacgtagtagcaaaagaacc |
ISG56 | ttgatgacgatgaaatgcctga | caggtcaccagactcctcac |
IFNAR2 | tcatggtgtatatcagcctcgt | agttggtacaatggagtggtttt |
Mx1 | gtttccgaagtggacatcgca | ctgcacaggttgttctcagc |
OAS | ctgatgcaggaactgtatagcac | cacagcgtctagcacctctt |
ISG15 | cgcagatcacccagaagatcg | ttcgtcgcatttgtccacca |
IFI44L | agccgtcagggatgtactataac | agggaatcatttggctctgtaga |
IFITM1 | ccaaggtccaccgtgattaac | atgaaccacattgtgcaaacct |
Apobec3G | gcatcgtgaccaggagtatga | gtcagggtaaccttcgggt |
β-actin | gccgggacctgactgacacctcat | tttgcggtggacgatggagg |