Fig. 4
From: Identification of universal and cell-type specific p53 DNA binding
Chromatin accessibility contributes to variable p53 binding. (A) Scatterplots illustrating the relationship between basal gene expression and p53 ChIP signal the 12 cell lines for four p53 peaks/genes. Note that in many cases, multiple cell lines show little gene expression or p53 binding and therefore cluster near the origin. (B) UCSC screen shots of two p53 binding sites; p53 binding in the proximity of GREB1 is found in MCF7 treated with IR, while IL1A was bound in IR treated LOXIMVI cells. ATAC-seq data and published DNase hypersensitivity data (for MCF7, untreated) showing that IR induced p53 binding correlates with basal DNA accessibility in each cell line. (C) Scatter plot of p53 binding post IR in MCF7 compared to LOXIMVI, colored by the difference in ATAC-seq signal: log2(LOXIMVI)-log2(MCF7) between the two cell lines