Fig. 5

Cellular state regulates p53 binding. (A) Scatter plot of p53 binding in IR treated MCF7 cells compared to MCF7 treated with IR and decitabine, colored by the difference in ATAC-seq signal between decitabine treated and untreated cells: log2(decitabine)-log2(untreated). (B) UCSC screen shot of the region around the gene DLGAP5, showing changes in p53 binding and accessibility in the decitabine treated MCF7 cells (the new peak is indicated by an arrow). Binding of p53 in IR treated UACC62 cells at the DLGAP5 locus without decitabine treatment is also shown. (C) UCSC screen shot of the IL1 locus showing increased p53 binding in TGFβ treated cells. (D) Boxplot showing log2 change in gene expression (TPM of TGFβ treated/untreated) in genes nearby p53 binding sites that showed altered occupancy in TGFβ treated cells. (E) Gene expression of IL1A and IL1B in cells treated as indicated with Nutlin3A or TGFβ (N = 3 experiments, except TGFβ + Nutlin3A N = 2). (F) Expression of IL1A and IL1B measured by RNA-seq in A549 cells treated with TGFβ comparing p53 knockdown cells to control knockdown (N = 3). All error bars are SEM