Fig. 4

a Analysis of evolutionarily conserved amino acid residues of GJA3 by ConSurf. The colour coding bar shows the conservation score, and boxes indicate the high-risk nsSNPs. b Distribution of functional, exposed sites (blue) and conserved, buried sites (orange) of high-risk nsSNPs of GJA3 predicted by ConSurf. c Distribution of high-risk nsSNPs of GJA3 in random coils, alpha helixes, extended strands and beta turns predicted by SOPMA. d SOPMA analysis of the secondary structure of individual amino acid residues in protein produced from the GJA3 gene. The boxes indicate the high-risk nsSNPs