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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Cathepsin K regulates localization and secretion of Tartrate-Resistant Acid Phosphatase (TRAP) in TRAP-overexpressing MDA-MB-231 breast cancer cells

Fig. 7

Cellular TRAP activity and wound migration after CtsK inhibition by MK0822. a Cells were treated with 10 nM and 100 nM CtsK inhibitor (MK-0822, Odanacatib), and lysates collected after 24 h of treatment. Total TRAP activity (represented by absorbance signal) from cell lysate derived from mock control or TRAP3high cells. Averages from n = 3 independent experiments were statistically compared by ANOVA test (Fisher’s LSD test). b Live cell wound migration of TRAP-overexpressing (TRAP3high, black) and control (ctrl, red) MDA-MB-231 cells under treatment with the CtsK inhibitor (10 nM and 100 nM of MK-0822, Odanacatib, independent experiments n = 3, 4 or more technical replicates each). Statistical comparison in GraphPad Prism 6 software was performed by ANOVA (Fisher’s LSD test) on migration velocity assessed by linear regression analysis between normally distributed groups. * p < 0.05, *** p < 0.001

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