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Table 2 Effect of temperature on transfection efficiency

From: Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

TemperatureViability (%)Fluorescence (%)
Room temperature13.4 ± 0.51 a7.3 ± 0.20 a
4 °C2.4 ± 0.24 b5.8 ± 0.15 b
  1. Other pulse conditions: 300 V; 1 pulse; pulse duration, 10 ms; 2 mm cuvette; 4 μg DNA; number of replicates = 3. The electroporation buffer was Opti-MEM with GlutaMax
  2. Different superscript letters indicate significant difference (P < 0.05)