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Table 3 Effect of cuvette type on transfection efficiency

From: Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

Cuvette type

Viability (%)

Fluorescent cells of vital cells (%)

2 mm

22.8 ± 1.1

17.2 ± 0.75

4 mm

61.0 ± 0.7

23.8 ± 0.61

  1. Other pulse conditions: 200 V; 1 pulse; pulse duration, 10 ms; 4 μg DNA; number of replicates = 3. The electroporation buffer was Opti-MEM with GlutaMax