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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth

Fig. 5

PGRMC1 phosphorylation status affects glucose uptake, lactate secretion, and mitochondrial function. a Glucose uptake by cell lines using the Cayman “Glycolysis” kit. The boxplots represent four technical replicates each for each of independent stably transfected sub-lines 1–3 of each condition WT, DM and TM (lines from Fig. 1). i.e. n = 4 × 3 = 12 per condition. For MP cells, 12 replicates of the MP cell line were performed. There was significant difference between all means (Kruskal-Wallis p < 0.0001, pairwise two-sample Kolmogorov-Smirnov Tests p < 0.0002). b Lactate secretion by cell lines. Details follow (A), except duplicates of each stable cell line were measured (n = 3 × 2 = 6 per condition). Inter cell-type comparison tests revealed that the means of all pairwise comparisons were significantly different from one another (ANOVA, post-hoc Dunnet’s T3, p < 0.003), except the WT-TM comparison which was not significant (p = 0.211). c The maximal respiratory capacity of mitochondria is affected by PGRMC1 phosphorylation status. Mitochondrial oxygen consumption of respective independent clonal stable lines C1 of each PGRMC1-HA mutant condition WT, DM and TM. Arrows indicate the time of addition of ATP synthase inhibitor oligomycin, Δψm uncoupler FCCP, and electron transport chain inhibitors rotenone & antimycin A. OCR: oxygen consumption rate (pmol/min normalized per μg protein), n = 5; mean +/− s.d.

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