Fig. 6
PGRMC1 phosphorylation influences mitochondrial and cell morphology together. a Images show cells with close to average form factor (avFF) values for elongate or rounded cells of each cell type. The numbers of cells scored in each class are indicated. All cell images are reproduced to the same scale. b Elongated and round cells from (a) examined according to cell type. The top left panel shows average percentage elongated cells scored for three independent biological replicate cell lines per cell type across all 3 replicates (n(Σ1–3)) corresponding to the given n values in (A). The remaining panels showing the distribution of cells in each shape and type category of Area, Perimeter and FF. Kruskal-Wallis analysis revealed no significant differences at the p < 0.05 level between any elongated cell type comparisons (ns), whereas all cell types exhibited significant differences for round cells (Kruskal-Wallis, P < 0.001). The accompanying Kruskal-Wallis post-hoc pairwise comparison p-values for round cells are given in the respective Tables. c AvFF per cell plotted including cell shape. The values where cells transition between round and elongated morphology (avFF 2.2–2.6) are indicated by dotted lines