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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Molecular effects of dADD1 misexpression in chromatin organization and transcription

Fig. 2

dADD1 overexpression induces HP1a delocalization, which correlates with loss of chromosome banding pattern at the chromocenter. a Classification of the severity of the phenotypes and the amount of HP1a signal observed in the polytene chromosomes. The first column indicates the genotypes analyzed; the second column shows the classification of the chromosomes according to their banding pattern in organized, moderately and severely affected (see text for a detailed explanation on the classification). Different shades of red indicate the intensity of HP1a signal. The third column shows an example of each of these polytene squashes where DNA is evidenced by DAPI staining (shown in gray), HP1a (red) scale bar 20 μm. b Area of HP1a signal quantified with ImageJ. The number of chromosomes analyzed for each condition is shown with “n,” an Unpaired t-test was performed to determine significance, the Area of HP1a signal is diminished in dADD1 overexpressing chromosomes (>dAdd1 vs. wt). c Correlation between HP1a and loss of chromosome banding pattern. Ordinary one-way ANOVA was performed to determine the significance, p < 0.0001****

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