Fig. 4
From: Molecular effects of dADD1 misexpression in chromatin organization and transcription
Overexpression of dADD1 proteins disturbs H3K9me3 signal. a Immunostaining of polytene chromosomes from wild-type, Sgs3-GAL4/UAS-dADD1a (>dAdd1a) and Sgs3-GAL4/UAS-dADD1b (>dADD1b), DNA (DAPI staining shown in grey), HP1a (red signal), H3K9me3 (green signal) and merge scale bar 20 μm. b The signal area of HP1a was quantified by ImageJ as well as the intensity of HP1a and H3K9me3 signal. The “n” represents the number of chromosomes analyzed in each genotype; an Ordinary one-way ANOVA test was performed to determine significance p < 0.05*, < 0.01 **, < 0.001***, < 0.0001****