Fig. 5

dADD1 overexpression results in transcriptional silencing of some genes and loss of HP1a. qRT-PCR analyses, wild-type (gray bars), null dADD1 (aqua bars), Sgs3-GAL4/UAS-dADD1a (red bars) and Sgs3-GAL4/UAS-dADD1b (dark blue bars). Three independent biological replicates were performed. Data are shown as expression relative to Rp49 transcript. Ordinary one-way ANOVA was executed to determine significance with P-values (p < 0.05*, < 0.01 **, < 0.001***, < 0.0001****, ns, no significance). a controls, b euchromatin genes, c genes regulated by Su(var)3–9, d genes regulated by HP1a, e genes regulated by Su(var)3–9 and HP1a, f pericentric genes. HP1a protein enrichment at different analyzed promoters. Chromatin immunoprecipitation experiments were performed using the C1A9 antibody (anti-HP1a) and mouse IgG as Mock. Psi, Sgs8 and Hsp70 are euchromatic regions without HP1a (g and h and n); note there is no enrichment versus the mock in the wild-type condition. Promoters of the Het-A retrotransposon (i) and Cin (j) were evaluated as telomeric and sub-telomeric regions in which HP1a is localized. Cta (k) and Lt (l) are pericentromeric, and HP1a also regulates them. Ank is regulated by Su (var)3–9 and HP1a (m). Error bars represent standard deviation. P-values (p < 0.05*, < 0.01 **, < 0.001***, < 0.0001****, ns, no significance)