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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Characterization of the interactions between Codanin-1 and C15Orf41, two proteins implicated in congenital dyserythropoietic anemia type I disease

Fig. 2

The C-terminal domain of Codanin-1 binds to C15Orf41 and elevates its levels. a. Schematic representation of Codanin-1 sub-fragments. The amino acid composition of each fragment is indicated to the right. FL – full-length protein. The arrowhead indicates the flag tag. b. Co-Immunoprecipitation of Codanin-1 fragments. HeLa cells were transfected with HA-C15orf41 and the indicated Codanin-1 fragments. Co-immunoprecipitation was performed using Flag antibody. Whole cell extracts (WCE) and co-immunoprecipitated proteins (CO-IP) are shown. Codanin-1 overexpression did not influence the levels of the endogenous ASF1 levels, and the N-terminus of Codanin-1 (F1) precipitated ASF1. c. C15Orf41 protein elevation is dependent on the levels of Codanin-1 overexpression. Western blot of HeLa cell lysate transfected with equivalent levels of HA-C15ORF41 and different concentrations of Codanin-1-Flag. β-tubulin was used as a loading control

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