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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Characterization of the interactions between Codanin-1 and C15Orf41, two proteins implicated in congenital dyserythropoietic anemia type I disease

Fig. 4

Codanin-1 overexpression shifts C15Orf41 protein from the nucleus to the cytoplasm. a-c. HeLa cells co-transfected with the indicated constructs were stained with DAPI (blue) and antibodies against HA (green) and Flag (red). The immunofluorescence visualization of the cells was performed with axioimager microscopy. d. Red - The ratio of Codanin-1 protein levels in the cytoplasm compared to the nucleus. The p-value of the ratio between Codanin-1 alone to co-transfection of Codanin-1 and C15Orf41 is 0.027. Green – The ratio of C15Orf41 protein levels in the cytoplasm compared to the nucleus. The p-value of the ratio between C15Orf41 alone to co-transfection of Codanin-1 and C15Orf41 < .00001. e. Cell fractionation following overexpression of C15Orf41 alone or co-expression of C15Orf41 and Codanin-1. The purity of the fractions was confirmed by probing with antibodies against A lamin (nuclear) and tubulin (cytoplasmic). Note that due to the instability of C15Orf41 when expressed alone (without Codanin-1), the levels of the transfected C15Orf41 DNA were three times higher than the transfected levels when it was co-transfected with Codanin-1

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