TY - JOUR AU - Gagné, David AU - Benoit, Yannick D. AU - Groulx, Jean-François AU - Vachon, Pierre H. AU - Beaulieu, Jean-François PY - 2020 DA - 2020/03/17 TI - ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process JO - BMC Molecular and Cell Biology SP - 14 VL - 21 IS - 1 AB - Fibronectin (FN) assembly into an insoluble fibrillar matrix is a crucial step in many cell responses to extracellular matrix (ECM) properties, especially with regards to the integrin-related mechanosensitive signaling pathway. We have previously reported that the silencing of expression of integrin-linked kinase (ILK) in human intestinal epithelial crypt (HIEC) cells causes significant reductions in proliferation and spreading through concomitantly acquired impairment of soluble FN deposition. These defects in ILK-depleted cells are rescued by growth on exogenous FN. In the present study we investigated the contribution of ILK in the fibrillogenesis of FN and its relation to integrin-actin axis signaling and organization. SN - 2661-8850 UR - https://doi.org/10.1186/s12860-020-00259-0 DO - 10.1186/s12860-020-00259-0 ID - Gagné2020 ER -