Fig. 2From: ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading processAdhesion to exogenous FN coating supports the restoration of the contractile phenotype in ILK-depleted HIEC. a IF microscopy images representative of the actin cytoskeleton (detected with TRITC-phalloidin; in red) and vinculin-positive ECM adhesion structures (anti-vinculin antibody; green) stained in siCNS and siILK cells grown for 24 h on serum-pretreated, FN-coated (FN; 3 μg/cm2) or Col-I-coated (Col-I;10 μg/cm2) glass coverslips. b Histograms of the average surface spreading area (μm2) measured numerically (Metamorph Imaging System software) on cells cultured under the same conditions as in (a). Results are expressed as the mean ± SEM (n = 3; > 50 cells have been analyzed for each condition). *** p < 0.001 in a one-way ANOVA using Tukey’s correction for multiple group comparisons. Scale bar in (a) 25 μmBack to article page