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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process

Fig. 4

Adhesion to exogenous FN/ECM-coated substrates restores DOC-insoluble FN assembly capacity in ILK-depleted HIEC. siCNS and siILK cells were grown for 36 to 48 h on uncoated, FN-coated (3 μg/cm2) or Col-I-coated (10 μg/cm2) plastic dishes and then harvested for DOC-insoluble proteins. The histograms present the levels of isolated human DOC-insoluble FN quantified from WB densitometry analysis for each condition. The β-actin levels detected in the corresponding soluble fractions were used to normalize the DOC-insoluble levels. Results are expressed as the percentage (%) of the siCNS cells on uncoated dishes ± SEM (n ≥ 9). *** p < 0.001 in a one-way ANOVA test using Tukey’s correction for multiple group comparisons. ns: not significant. The lower panel shows representative protein immunoblots of human DOC-insoluble (DOC-i.) FN, as well as corresponding ILK and β-actin, detected from the DOC-soluble fractions. b Inverted contrast microscopy images representative of the same cells grown for 24 h on the same uncoated and coated plastic dish culture conditions. Scale bar in (b) 30 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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