Fig. 6

Inhibition of FN synthesis with cycloheximide prevents FN fibrillogenesis in ILK-depleted HIEC on exogenous FN. (a, b and c) siCNS and siILK cells were grown for 24 h on uncoated or FN-coated plastic dishes, with or without cycloheximide (10 μg/ml) and were harvested for DOC-insoluble proteins. a Representative WB of human FN (HFN 7.1 antibody) detected in DOC-soluble (DOC-s.) and DOC-insoluble (DOC-i.) fractions, as well as the corresponding ILK and β-actin protein immunoblots detected from the DOC-s. fractions. The order of WB bands in (a) follows the order of the histograms presented in (b and c). (b and c) Histograms of the levels of human FN quantified from WB densitometry analysis of siCNS and siILK cells b DOC-soluble and c DOC-insoluble fractions. β-actin detected in the soluble fractions was used to normalize the results for each experiment. Results are expressed as the percentage (%) of the siCNS cells on uncoated dishes ± SEM. (n = 4). *** p < 0.001, ** p < 0.01 and * p < 0.05 in a one-way ANOVA test using Newman-Keuls correction for multiple group comparisons. ns: not significant