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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process

Fig. 7

Effect of ILK silencing on α5β1 integrin, FN, and tensin distribution in HIEC. (a and b) siCNS and siILK cells were grown for 24 h on serum-pretreated glass coverslips and then fixed and prepared for IF staining. a Co-IF microscopy images representative of α5β1 integrin (ITGA5; α5 subunit detected with the AB1928 antibody) and cell-associated human FN (HFN 7.1 antibody) staining distribution in siCNS and siILK cells. The right panels show merged and magnified co-detection (ITGA5 in green, FN red, DAPI staining of the nucleus in blue). White arrows in siCNS points to ITGA5 and FN colocalization in fibrillar structures. Red arrows and red arrowheads in siILK point respectively to subcortical staining of α5 integrin and its colocalization with small punctuated aggregates of FN. b Co-IF microscopy images representative of tensin (green) and vinculin (red) staining distribution in siCNS and siILK cells. Scale bar in (a): 10 μm; (b): 5 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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