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Fig. 8 | BMC Molecular and Cell Biology

Fig. 8

From: ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process

Fig. 8

Adhesion to exogenous FN restores normal α5β1 and αVβ3 integrin distribution in ILK-depleted HIEC. siCNS and siILK cells were grown for 24 h on serum-pretreated or FN-coated glass coverslips and then fixed and prepared for IF staining. (a and b) Co-IF microscopy images representative of α5β1 integrin (ITGA5; α5 subunit/AB1928 antibody) staining with a ILK staining, and b αVβ3 staining distribution in siCNS and siILK cells. Scale bar in (a and b): 15 μm

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