Fig. 9

Effect of α5 or αV integrins and concomitant ILK silencing on FN assembly in HIEC. (a and b) HIEC cells were transfected with 20 nM of siCNS, siILK or siRNA directed against the expression of α5 (siITGA5) and αV (siITGAV) integrin subunits alone or in combination with 20 nM of siILK (e.g. siITGA5/siILK). The cells were subsequently plated and grown for 48 h on uncoated, FN-coated and Col-I coated plastic dishes before being harvested for DOC-insoluble protein extraction. a The histograms present the levels of human DOC-insoluble FN quantified from densitometry analysis of WB bands. β-actin detected in the soluble fractions was used to normalize the results for each experiment. Results are expressed as the percentage (%) of the siCNS on uncoated dishes ± SEM (n ≥ 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 in a one-way ANOVA test using Tukey’s correction for multiple group comparisons (only the statistically significant differences are indicated). b Representative protein immunoblots of DOC-insoluble (DOC-i.) human FN and the corresponding ILK, β-actin, α5 (ITGA5) and αV (ITGAV) integrin subunits detected from the DOC-soluble fractions of siCNS, siILK, siITGA5/siILK and siITGAV/siILK cells