Fig. 1
From: BACE2 degradation is mediated by both the proteasome and lysosome pathways
The half-life of transiently overexpressed BACE2 is approximately 6 h in HEK293 cells. a HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 100 μg/mL CHX for 0, 2, 4, 8, 12 and 16 h, respectively. Cell lysates were resolved by 10% SDS-PAGE. BACE2 expression was detected by using 9E10. β-actin was detected by AC-15 and served as an internal reference. b Quantification of BACE2 levels at each time-point. BACE2 protein levels were plotted as a percentage of the amount of BACE2 level at 0 h. Values are mean ± SEM; n ≥ 3