Fig. 2

Lysosomal inhibition causes a time- and dose-dependent increase of transiently overexpressed BACE2 in HEK293 cells. a HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 0, 10, 25 or 50 mM lysosome inhibitor NH₄CL for 24 h. b Quantification of BACE2 levels. c HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 25 mM NH₄CL for 0, 6, 12 or 24 h. d Quantification of BACE2 levels. e HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 0, 10, 100 or 300 nM Baf-A1 for 24 h. f Quantification of BACE2 levels. g HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with Baf-A1 for 0, 4, 12 or 24 h. (h) Quantification of BACE2 levels. 9E10 antibody was used to detect myc-tagged BACE2 protein. β-actin served as an internal control. Values represent mean ± SEM; n ≥ 3, *P < 0.05 by one-way ANOVA followed by Tukey’s test