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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics

Fig. 1

Isolation of nucleic acid-associated proteins. Formaldehyde was introduced into bacterial cultures to stabilize protein-nucleic acid interactions through crosslinking. Bacterial cells were lysed and protein-DNA complexes fragmented prior to immunoprecipitation using an anti-RNA polymerase antibody immobilized on protein G coated magnetic beads. Immunoprecipitated proteins were digested of magnetic beads using trypsin and mass spectrometry analysis was performed to identify nucleic acid-associated proteins

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