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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics

Fig. 6

Immunoprecipitation of N-terminal FLAG-tagged M. smegmatis proteins. FLAG-tagged MSMEG_1060, MSMEG_2695, MSMEG_3754, MSMEG_4306 and MSMEG_5512 were episomally expressed in M. smegmatis. The first lane of each gel contains a GeneRuler™ 1 kb plus DNA molecular weight marker (MW), − and + indicates the absence or presence of the anti-FLAG-tag antibody. Negative controls, FLAG only and FLAG-EccA3, did not demonstrate any association with DNA following immunoprecipitation. The positive control FLAG-HupB, FLAG-MSMEG_1060, FLAG-MSMEG_2695 and FLAG-MSMEG_4306 did demonstrate DNA association following immunoprecipitation

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