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Table 1 AP-MS identified proteins selected for validation

From: Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics

MSMEG Gene Annotation M. tuberculosis Orthologue annotationa Protein Namesb Protein Domainb,c,d Descriptionc,d
MSMEG_1060 Putative Lsr2 protein (Uncharacterized protein) Lsr2 is a DNA-bridging protein in Mycobacterium.
MSMEG_2695 Rv2744c 35 kDa protein PspA/IM30 PspA suppresses sigma54-dependent transcription, negative regulator of E. coli phage shock operon.
MSMEG_3754 Rv1691 Tetratricopeptide repeat (TPR)-repeat-containing protein TPR TPRs have shown involvement in cell cycle regulation, transcriptional control, and protein folding.
MSMEG_4306 Uncharacterized protein C4-type zinc ribbon Structural modelling suggests that Zn-ribbon domain may bind nucleic acids.
MSMEG_5512 Rv0958 Magnesium Chelatase RNA polymerase
sigma factor 54 interaction domain
Interaction with sigma-54 factor and has ATPase activity. Half of the proteins identified with this domain might belong to signal transduction two-component systems.
  1. a Obtained from Mycobrowser (https://mycobrowser.epfl.ch/), b Obtained from Uniprot (https://www.uniprot.org/), c Obtained from InterPro (https://www.ebi.ac.uk/interpro/), d Obtained from Pfam (https://pfam.xfam.org/). PspA/IM30 domain first identified in the PspA protein in Escherichia coli [31], Tetratricopeptide repeat (TPR) have been identified in a wide variety of proteins including transcription factors [32], structural modelling suggests nucleic acid binding by C4-type zinc ribbon [33]