Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics

Table 1 AP-MS identified proteins selected for validation

MSMEG Gene Annotation	M. tuberculosis Orthologue annotation^a	Protein Names^b	Protein Domain^b,c,d	Description^c,d
MSMEG_1060	–	Putative Lsr2 protein (Uncharacterized protein)	–	Lsr2 is a DNA-bridging protein in Mycobacterium.
MSMEG_2695	Rv2744c	35 kDa protein	PspA/IM30	PspA suppresses sigma54-dependent transcription, negative regulator of E. coli phage shock operon.
MSMEG_3754	Rv1691	Tetratricopeptide repeat (TPR)-repeat-containing protein	TPR	TPRs have shown involvement in cell cycle regulation, transcriptional control, and protein folding.
MSMEG_4306	–	Uncharacterized protein	C4-type zinc ribbon	Structural modelling suggests that Zn-ribbon domain may bind nucleic acids.
MSMEG_5512	Rv0958	Magnesium Chelatase	RNA polymerase sigma factor 54 interaction domain	Interaction with sigma-54 factor and has ATPase activity. Half of the proteins identified with this domain might belong to signal transduction two-component systems.

^a Obtained from Mycobrowser (https://mycobrowser.epfl.ch/), ^b Obtained from Uniprot (https://www.uniprot.org/), ^c Obtained from InterPro (https://www.ebi.ac.uk/interpro/), ^d Obtained from Pfam (https://pfam.xfam.org/). PspA/IM30 domain first identified in the PspA protein in Escherichia coli [31], Tetratricopeptide repeat (TPR) have been identified in a wide variety of proteins including transcription factors [32], structural modelling suggests nucleic acid binding by C4-type zinc ribbon [33]

ISSN: 2661-8850