Fig. 7
From: Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades
Effects of compounds in the Uba1~ubiquitin thioester formation Alpha assay. Data represent mean and SD for 3–5 samples in each case. Final DMSO concentration was 1% in all cases. FLAG-Uba1 samples were preincubated with compounds for 15 min prior to addition of biotin-ubiquitin and ATP. a Dose–response for bioactive inhibitors of Uba1~ubiquitin thioester formation, plotted both linearly and semi-logarithmically (inset). b Effects of compounds with little activity against Uba1~ubiquitin thioester formation at 500 μM, normalized to positive (DMSO alone) control values. Calculated IC50 values for the compounds are shown in Table 1