Table 1 Half-maximal inhibitory concentration (IC₅₀) values for compounds against PCNA ubiquitination and Uba1~ubiquitin thioester formation by the Alpha assay compared to other methods. All IC₅₀, standard error (SE), and 95% confidence interval (CI) values listed were calculated by nonlinear regression with GraphPad Prism software. Ind. = indeterminate

EGCG

0.0566 μM (SE = 0.00954; CI: 0.0409–0.0776)

0.228 μM (SE = 0.0342; CI: 0.171–0.319)

0.488 μM (SE = 0.145; CI: 0.259–0.949)

1.63 μM (SE = 0.458; CI:

0.835–3.45)

ECG

0.0988 μM (SE = 0.0442; CI: 0.0318–0.275)

0.537 μM (SE = 0.0946; CI: 0.375–0.843)

0.769 μM (SE = 0.113; CI: 0.586–1.02)

4.22 μM (SE =

2.16; CI: 1.26–13.78)

EGC

6.62 μM (SE = 3.23; CI: 2.92–16.5)

43.3 μM (SE = 22.8; CI: 16.1–129)

5.96 μM (SE = 17.3; CI: 0.101–Ind.)

7.58 μM (SE = 3.87; CI: 2.93–20.7)

EC

>  500 μM

>  500 μM

>  500 μM

>  500 μM

Cat

ca. 500 μM

>  500 μM

>  500 μM

>  500 μM

GA

>  500 μM

>  500 μM

>  500 μM

>  500 μM

PG

>  500 μM

>  500 μM

>  500 μM

>  500 μM

OG

3.08 μM (SE = 0.611; CI: 2.59–6.35)

10.3 μM (SE = 6.41; CI: 3.18–40.1)

13.4 μM (SE = 6.02; CI: 5.34–38.5)

80.6 μM (SE = 33.9; CI: 34.8–405)

DG

3.73 μM (SE = 0.566; CI: 2.83–4.56)

1.63 μM (SE = 0.312; CI: 1.05–2.67)

44.4 μM (SE = 22.4; CI: 18.8–150)

35.0 μM (SE = 25.1; CI: 6.29–533)

Myr

0.760 μM (SE = 0.393; CI: 0.323–2.61)

1.21 μM (SE = 0.208; CI: 0.876–4.20)

1.59 μM (SE = 0.746; CI: 0.721–4.22)

0.721 μM (SE = 0.497; CI: 0.177–2.68)

DM

28.7 μM (SE = 5.30; CI: 19.7–49.5)

66.4 μM (SE = 38.9; CI: 27.2–181)

27.5 μM (SE = 13.6; CI: 7.52–113)

14.3 μM (SE = 3.14; CI: 5.93–22.2)