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Table 1 Half-maximal inhibitory concentration (IC50) values for compounds against PCNA ubiquitination and Uba1~ubiquitin thioester formation by the Alpha assay compared to other methods. All IC50, standard error (SE), and 95% confidence interval (CI) values listed were calculated by nonlinear regression with GraphPad Prism software. Ind. = indeterminate

From: Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades

CompoundIC50for inhibition of PCNA ubiquitination (Alpha)IC50for inhibition of PCNA ubiquitination
(Western blot) [19]
IC50for inhibition of Uba1~Ub thioester formation
(Alpha)
IC50for inhibition of Uba1~Ub thioester formation (gel-based) [19]
EGCG0.0566 μM (SE = 0.00954; CI: 0.0409–0.0776)0.228 μM (SE = 0.0342; CI: 0.171–0.319)0.488 μM (SE = 0.145; CI: 0.259–0.949)1.63 μM (SE = 0.458; CI:
0.835–3.45)
ECG0.0988 μM (SE = 0.0442; CI: 0.0318–0.275)0.537 μM (SE = 0.0946; CI: 0.375–0.843)0.769 μM (SE = 0.113; CI: 0.586–1.02)4.22 μM (SE =
2.16; CI: 1.26–13.78)
EGC6.62 μM (SE = 3.23; CI: 2.92–16.5)43.3 μM (SE = 22.8; CI: 16.1–129)5.96 μM (SE = 17.3; CI: 0.101–Ind.)7.58 μM (SE = 3.87; CI: 2.93–20.7)
EC>  500 μM>  500 μM>  500 μM>  500 μM
Catca. 500 μM>  500 μM>  500 μM>  500 μM
GA>  500 μM>  500 μM>  500 μM>  500 μM
PG>  500 μM>  500 μM>  500 μM>  500 μM
OG3.08 μM (SE = 0.611; CI: 2.59–6.35)10.3 μM (SE = 6.41; CI: 3.18–40.1)13.4 μM (SE = 6.02; CI: 5.34–38.5)80.6 μM (SE = 33.9; CI: 34.8–405)
DG3.73 μM (SE = 0.566; CI: 2.83–4.56)1.63 μM (SE = 0.312; CI: 1.05–2.67)44.4 μM (SE = 22.4; CI: 18.8–150)35.0 μM (SE = 25.1; CI: 6.29–533)
Myr0.760 μM (SE = 0.393; CI: 0.323–2.61)1.21 μM (SE = 0.208; CI: 0.876–4.20)1.59 μM (SE = 0.746; CI: 0.721–4.22)0.721 μM (SE = 0.497; CI: 0.177–2.68)
DM28.7 μM (SE = 5.30; CI: 19.7–49.5)66.4 μM (SE = 38.9; CI: 27.2–181)27.5 μM (SE = 13.6; CI: 7.52–113)14.3 μM (SE = 3.14; CI: 5.93–22.2)